Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds

Nutrients. 2020 Apr 29;12(5):1261. doi: 10.3390/nu12051261.


Koreans have been consuming Petasites Japonicus (PJ) as food. Although the therapeutic effect of PJ on allergic or inflammatory reactions associated with asthma has been proven, its effect on obesity is unclear. Therefore, the present study was aimed to assess the obesity related anti-inflammatory and anti-adipogenic effects of ethanol extract PJ (EPJ) on the inflammatory response in RAW 264.7 macrophages and on differentiation in 3T3-L1 adipocytes. In addition, the polyphenolic compound was quantitatively characterized from the EPJ using ultra performance liquid chromatography coupled with diode array detector, quadrupole time-of-flight-mass spectrometry (UPLC-DAD-QToF-MS). In RAW 264.7 or 3T3-L1, reduction of nitric oxide (in macrophages) production as well as monocyte chemoattractant protein-1 and tumor necrosis factor-α were observed. Treatment of EPJ in adipocyte differentiation showed an improvement in adiponectin and lipid accumulation and a significant reduction of PPARγ and FABP-4 mRNA expression levels. On the other hand, mRNA expression of UCP-1, PPARα, and ACO increased in the EPJ treated group. In addition, a total of 26 polyphenolic compounds were detected and of which 12 are reported for the first time from PJ. The higher content of diverse polyphenolic compounds presented in EPJ might be responsible for the observed anti-inflammatory and anti-adipogenic effect. These results suggest that PJ is valuable in improving obesity-related inflammatory responses.

Keywords: Petasites japonicus; UPLC-DAD-QToF-MS; anti-adipogenic effects; anti-inflammation; anti-lipogenesis; characterization; flavonoids; ployphenolic compounds.

MeSH terms

  • 3T3 Cells
  • Adipocytes / metabolism*
  • Adipogenesis / drug effects*
  • Animals
  • Anti-Inflammatory Agents*
  • Anti-Obesity Agents*
  • Chemokine CCL2 / metabolism
  • Ethanol
  • Fatty Acid-Binding Proteins / metabolism
  • Macrophages / metabolism*
  • Mice
  • Nitric Oxide / metabolism
  • PPAR gamma / metabolism
  • Petasites / chemistry*
  • Plant Extracts / analysis*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Polyphenols / analysis*
  • Polyphenols / isolation & purification
  • Polyphenols / pharmacology*
  • RAW 264.7 Cells
  • Tumor Necrosis Factor-alpha / metabolism


  • Anti-Inflammatory Agents
  • Anti-Obesity Agents
  • CCL2 protein, human
  • Chemokine CCL2
  • FABP4 protein, human
  • Fatty Acid-Binding Proteins
  • PPAR gamma
  • Plant Extracts
  • Polyphenols
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • Ethanol