Taxotere-induced WNT16 Expression in Carcinoma-Associated Fibroblasts Might Associate with Progression and Chemoresistance of Breast Cancer

Ann Clin Lab Sci. 2020 Mar;50(2):205-212.

Abstract

Objective: The objective of this study is to investigate the relationship between WNT16 expression in carcinoma-associated fibroblasts and Taxotere treatments in breast cancer.

Procedures: WNT16 expression in primarily cultured carcinoma-associated fibroblasts was detected via Real-Time PCR and Western blot. A total of 106 breast cancer cases were prepared for immunohistochemistry. Multiple assays were performed to examine the function of breast cancer cells (MDA-MB-231) co-cultured with carcinoma-associated fibroblasts when WNT16 was inhibited.

Results: Enhanced WNT16 expression was verified in carcinoma-associated fibroblasts after Taxotere treatment (p<0.0001). MDA-MB-231 cells co-cultured with carcinoma-associated fibroblasts displayed decreasing invasion, proliferation abilities, and an increasing sensitivity to Taxotere treatment when WNT16 was inhibited. The WNT16 expression of carcinoma-associated fibroblasts in breast cancer was significantly associated with tumor size (p=0.0164), and chemotherapy treatment (p=0.0015).

Conclusions: The results indicate that Taxotere-induced WNT16 expression in carcinoma-associated fibroblasts might contribute to the progression and chemoresistance of breast cancer.

Keywords: WNT16; breast cancer; carcinoma-associated fibroblasts; chemoresistance.

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Biomarkers, Tumor / metabolism*
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Cancer-Associated Fibroblasts / metabolism
  • Cancer-Associated Fibroblasts / pathology*
  • Docetaxel / pharmacology*
  • Female
  • Follow-Up Studies
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Middle Aged
  • Neoadjuvant Therapy / mortality*
  • Prognosis
  • Receptor, ErbB-2 / metabolism
  • Receptors, Estrogen / metabolism
  • Receptors, Progesterone / metabolism
  • Survival Rate
  • Wnt Proteins / metabolism*

Substances

  • Antineoplastic Agents
  • Biomarkers, Tumor
  • Receptors, Estrogen
  • Receptors, Progesterone
  • WNT16 protein, human
  • Wnt Proteins
  • Docetaxel
  • ERBB2 protein, human
  • Receptor, ErbB-2