doi: 10.1039/d0cc02140g.
Epub 2020 May 5.
Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation
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- PMID: 32369063
- DOI: 10.1039/d0cc02140g
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Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation
Chem Commun (Camb).
.
Abstract
Circular RNA without a stop codon enables rolling circle translation. To produce circular RNAs, we carried out one-pot chemical synthesis of circular RNA from RNA fragments with the use of an EDC/HOBt-based chemical ligation reaction. The synthesized circular RNAs acted as translation templates, despite the presence of unnatural phosphoramidate linkages.
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