Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems

Appl Microbiol Biotechnol. 2020 Jul;104(13):5673-5688. doi: 10.1007/s00253-020-10640-w. Epub 2020 May 6.


Human tissue plasminogen activator was the first recombinant therapy protein that successfully produced in Chinese hamster ovary cells in 1986 and approved for clinical use. Since then, more and more therapeutic proteins are being manufactured in mammalian cells, and the technologies for recombinant protein production in this expression system have developed rapidly, with the optimization of both upstream and downstream processes. One of the most promising strategies is expression vector cassette optimization based on the expression vector cassette. In this review paper, these approaches and developments are summarized, and the future strategy on the utilizing of expression cassettes for the production of recombinant therapeutic proteins in mammalian cells is discussed.

Keywords: Cis-acting epigenetic regulatory elements; Mammalian cell culture; Recombinant therapeutic protein; Vector optimization.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Line
  • Epigenesis, Genetic
  • Gene Expression
  • Genetic Engineering
  • Genetic Vectors / genetics*
  • Humans
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / therapeutic use
  • Regulatory Elements, Transcriptional


  • Recombinant Proteins