Participation of TRPV1 in the activity of the GnRH system in male rats

doi:10.1111/ejn.14770

. 2020 Aug;52(3):2995-3001.

doi: 10.1111/ejn.14770. Epub 2020 May 20.

Participation of TRPV1 in the activity of the GnRH system in male rats

Pablo Nicolás Surkin^{1

2}, Ganna Dmytrenko³, Noelia Paula Di Giorgio⁴, Marianne Bizzozzero⁴, Andrea De Laurentiis³, Javier Fernández-Solari^{1

2}

Affiliations

¹ Facultad de Odontología, Cátedra de Fisiología, Universidad de Buenos Aires, Buenos Aires, Argentina.
² Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
³ Centro de Estudios Farmacológicos y Botánicos, Facultad de Medicina, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Buenos Aires, Argentina.
⁴ Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.

PMID: 32372526
DOI: 10.1111/ejn.14770

Participation of TRPV1 in the activity of the GnRH system in male rats

Pablo Nicolás Surkin et al. Eur J Neurosci. 2020 Aug.

. 2020 Aug;52(3):2995-3001.

doi: 10.1111/ejn.14770. Epub 2020 May 20.

Authors

Pablo Nicolás Surkin^{1

2}, Ganna Dmytrenko³, Noelia Paula Di Giorgio⁴, Marianne Bizzozzero⁴, Andrea De Laurentiis³, Javier Fernández-Solari^{1

2}

Affiliations

¹ Facultad de Odontología, Cátedra de Fisiología, Universidad de Buenos Aires, Buenos Aires, Argentina.
² Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
³ Centro de Estudios Farmacológicos y Botánicos, Facultad de Medicina, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Buenos Aires, Argentina.
⁴ Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.

PMID: 32372526
DOI: 10.1111/ejn.14770

Abstract

GnRH neuron activity is under the influence of multiple stimuli, including those coming from the endocannabinoid and the immune systems. Since it has been previously suggested that some of the main elements controlling the GnRH pulse generator possess the TRPV1 receptor, the aim of the present study was to evaluate the participation of the hypothalamic TRPV1, through its pharmacological blockade, in the activity of the hypothalamic-pituitary-testicular axis in male rats under basal or acute inflammatory conditions. Our hypothesis was based on the idea that the hypothalamic TRPV1 participates in the synthesis of the main neuromodulatory signals controlling GnRH, and therefore the reproductive axis. Our results showed that the hypothalamic TRPV1 blockade induced pro-inflammatory effects by increasing Tnfα and Il-1β mRNA hypothalamic levels and inhibited the reproductive axis by affecting Gnrh, Kiss1 and Rfrp3 mRNA levels and decreasing plasma levels of luteinizing hormone and testosterone under basal conditions, without significant additive effects in rats exposed to systemic LPS. Altogether, these results suggest that the hypothalamic TRPV1 receptor participates in the regulation of the GnRH system, probably by modulating immune-dependent mechanisms.

Keywords: GnRH; RFRP-3; endocannabinoids; hypothalamus; kisspeptin.

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References

REFERENCES

1. Boychuk, C. R., Zsombok, A., Tasker, J. G., & Smith, B. N. (2013). Rapid glucocorticoid-induced activation of TRP and CB1 receptors causes biphasic modulation of glutamate release in gastric-related hypothalamic preautonomic neurons. Frontiers in Neuroscience, 7, 3. https://doi.org/10.3389/fnins.2013.00003
1. Brown, C. H., Bains, J. S., Ludwig, M., & Stern, J. E. (2013). Physiological regulation of magnocellular neurosecretory cell activity: Integration of intrinsic, local and afferent mechanisms. Journal of Neuroendocrinology, 25(8), 678-710. https://doi.org/10.1111/jne.12051
1. Castellano, J. M., Bentsen, A. H., Romero, M., Pineda, R., Ruiz-Pino, F., Garcia-Galiano, D., … Tena-Sempere, M. (2010). Acute inflammation reduces kisspeptin immunoreactivity at the arcuate nucleus and decreases responsiveness to kisspeptin independently of its anorectic effects. American Journal of Physiology. Endocrinology and Metabolism, 299(1), E54-61. https://doi.org/10.1152/ajpendo.00081.2010
1. Cavanaugh, D. J., Chesler, A. T., Jackson, A. C., Sigal, Y. M., Yamanaka, H., Grant, R., … Basbaum, A. I. (2011). Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells. Journal of Neuroscience, 31(13), 5067-5077. https://doi.org/10.1523/JNEUROSCI.6451-10.2011
1. De Laurentiis, A., Araujo, H. A., & Rettori, V. (2014). Role of the endocannabinoid system in the neuroendocrine responses to inflammation. Current Pharmaceutical Design, 20(29), 4697-4706.

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[1] Boychuk, C. R., Zsombok, A., Tasker, J. G., & Smith, B. N. (2013). Rapid glucocorticoid-induced activation of TRP and CB1 receptors causes biphasic modulation of glutamate release in gastric-related hypothalamic preautonomic neurons. Frontiers in Neuroscience, 7, 3. https://doi.org/10.3389/fnins.2013.00003

[2] Boychuk, C. R., Zsombok, A., Tasker, J. G., & Smith, B. N. (2013). Rapid glucocorticoid-induced activation of TRP and CB1 receptors causes biphasic modulation of glutamate release in gastric-related hypothalamic preautonomic neurons. Frontiers in Neuroscience, 7, 3. https://doi.org/10.3389/fnins.2013.00003

[3] Brown, C. H., Bains, J. S., Ludwig, M., & Stern, J. E. (2013). Physiological regulation of magnocellular neurosecretory cell activity: Integration of intrinsic, local and afferent mechanisms. Journal of Neuroendocrinology, 25(8), 678-710. https://doi.org/10.1111/jne.12051

[4] Brown, C. H., Bains, J. S., Ludwig, M., & Stern, J. E. (2013). Physiological regulation of magnocellular neurosecretory cell activity: Integration of intrinsic, local and afferent mechanisms. Journal of Neuroendocrinology, 25(8), 678-710. https://doi.org/10.1111/jne.12051

[5] Castellano, J. M., Bentsen, A. H., Romero, M., Pineda, R., Ruiz-Pino, F., Garcia-Galiano, D., … Tena-Sempere, M. (2010). Acute inflammation reduces kisspeptin immunoreactivity at the arcuate nucleus and decreases responsiveness to kisspeptin independently of its anorectic effects. American Journal of Physiology. Endocrinology and Metabolism, 299(1), E54-61. https://doi.org/10.1152/ajpendo.00081.2010

[6] Castellano, J. M., Bentsen, A. H., Romero, M., Pineda, R., Ruiz-Pino, F., Garcia-Galiano, D., … Tena-Sempere, M. (2010). Acute inflammation reduces kisspeptin immunoreactivity at the arcuate nucleus and decreases responsiveness to kisspeptin independently of its anorectic effects. American Journal of Physiology. Endocrinology and Metabolism, 299(1), E54-61. https://doi.org/10.1152/ajpendo.00081.2010

[7] Cavanaugh, D. J., Chesler, A. T., Jackson, A. C., Sigal, Y. M., Yamanaka, H., Grant, R., … Basbaum, A. I. (2011). Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells. Journal of Neuroscience, 31(13), 5067-5077. https://doi.org/10.1523/JNEUROSCI.6451-10.2011

[8] Cavanaugh, D. J., Chesler, A. T., Jackson, A. C., Sigal, Y. M., Yamanaka, H., Grant, R., … Basbaum, A. I. (2011). Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells. Journal of Neuroscience, 31(13), 5067-5077. https://doi.org/10.1523/JNEUROSCI.6451-10.2011

[9] De Laurentiis, A., Araujo, H. A., & Rettori, V. (2014). Role of the endocannabinoid system in the neuroendocrine responses to inflammation. Current Pharmaceutical Design, 20(29), 4697-4706.

[10] De Laurentiis, A., Araujo, H. A., & Rettori, V. (2014). Role of the endocannabinoid system in the neuroendocrine responses to inflammation. Current Pharmaceutical Design, 20(29), 4697-4706.

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Participation of TRPV1 in the activity of the GnRH system in male rats

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Participation of TRPV1 in the activity of the GnRH system in male rats

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