Applying MAPPs Assays to Assess Drug Immunogenicity

Front Immunol. 2020 Apr 21:11:698. doi: 10.3389/fimmu.2020.00698. eCollection 2020.

Abstract

Immunogenicity against biotherapeutic proteins (BPs) and the potential outcome for the patient are difficult to predict. In vitro assays that can help to assess the immunogenic potential of BPs are not yet used routinely during drug development. MAPPs (MHC-associated peptide proteomics) is one of the assays best characterized regarding its value for immunogenicity potential assessment. This review is focusing on recent studies that have employed human HLA class II-MAPPs assays to rank biotherapeutic candidates, investigate clinical immunogenicity, and understand mechanistic root causes of immunogenicity. Advantages and challenges of the technology are discussed as well as the different areas of application.

Keywords: ABIRISK; HLA; MAPPs; biotherapeutics; dendritic cells; drug development; immunogenicity; proteomics.

Publication types

  • Review

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / therapeutic use
  • Antigen Presentation
  • Biological Products / immunology*
  • Biological Products / therapeutic use
  • Drug Development
  • Epitopes, T-Lymphocyte / immunology
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Proteins / immunology*
  • Proteins / therapeutic use
  • Proteomics / methods*
  • T-Lymphocytes / immunology

Substances

  • Antibodies, Monoclonal
  • Biological Products
  • Epitopes, T-Lymphocyte
  • Histocompatibility Antigens Class II
  • Proteins