Saccharopolyspora coralli sp. nov. a novel actinobacterium isolated from the stony coral Porites

Abstract

A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated strain E2A^T, was isolated from a coral sample and examined using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E2A^T formed a distinct phyletic lineage in the genus Saccharopolyspora and was closely related to S. cavernae CCTCC AA 2012022^T (96.4 %) and S. lacisalsi CCTCC AA 2010012^T (95.3 %). The isolate grew at 15-35 °C, pH 5-12 and in the presence of 1-16 % (w/v) NaCl. The cell-wall diamino acid was meso-DAP. Major fatty acids identified were iso-C_{15 : 0}, iso-C_{16 : 0} and C_{17 : 1} ω8c. The predominant menaquinone was MK-9(H₄). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified glycolipid, one unidentified phospholipid and one unidentified aminolipid. The genomic DNA G+C content was 68.6 mol%. Based on the data from the polyphasic taxonomic study reported here, strain E2A^T represents a novel species within the genus Saccharopolyspora, for which the name Saccharopolyspora coralli sp. nov. is proposed. The type strain is E2A^T=(JCM 31844^T=MCCC 1A17150^T).

Keywords: Saccharopolyspora coralli sp. nov.; polyphasic taxonomy; stony coral.