The sodium channel NaV 1.5 impacts on early murine embryonic cardiac development, structure and function in a non-electrogenic manner

Gerard A Marchal; Arie O Verkerk; Rajiv A Mohan; Rianne Wolswinkel; Bastiaan J D Boukens; Carol Ann Remme

doi:10.1111/apha.13493

The sodium channel Na_V 1.5 impacts on early murine embryonic cardiac development, structure and function in a non-electrogenic manner

Acta Physiol (Oxf). 2020 Oct;230(2):e13493. doi: 10.1111/apha.13493. Epub 2020 May 27.

Authors

Gerard A Marchal¹, Arie O Verkerk^{1

2}, Rajiv A Mohan^{1

2}, Rianne Wolswinkel¹, Bastiaan J D Boukens², Carol Ann Remme¹

Affiliations

¹ Department of Experimental Cardiology, Amsterdam UMC (location Academic Medical Center), Amsterdam, the Netherlands.
² Department of Medical Biology, Amsterdam UMC (location Academic Medical Center), Amsterdam, the Netherlands.

Abstract

Aim: The voltage-gated sodium channel Na_V 1.5, encoded by SCN5A, is essential for cardiac excitability and ensures proper electrical conduction. Early embryonic death has been observed in several murine models carrying homozygous Scn5amutations. We investigated when sodium current (I_Na ) becomes functionally relevant in the murine embryonic heart and how Scn5a/Na_V 1.5 dysfunction impacts on cardiac development.

Methods: Involvement of Na_V 1.5-generated I_Na in murine cardiac electrical function was assessed by optical mapping in wild type (WT) embryos (embryonic day (E)9.5 and E10.5) in the absence and presence of the sodium channel blocker tetrodotoxin (30 µmol/L). I_Na was assessed by patch-clamp analysis in cardiomyocytes isolated from WT embryos (E9.5-17.5). In addition, cardiac morphology and electrical function was assessed in Scn5a-1798insD^-/- embryos (E9.5-10.5) and their WT littermates.

Results: In WT embryos, tetrodotoxin did not affect cardiac activation at E9.5, but slowed activation at E10.5. Accordingly, patch-clamp measurements revealed that I_Na was virtually absent at E9.5 but robustly present at E10.5. Scn5a-1798insD^-/- embryos died in utero around E10.5, displaying severely affected cardiac activation and morphology. Strikingly, altered ventricular activation was observed in Scn5a-1798insD^-/- E9.5 embryos before the onset of I_Na , in addition to reduced cardiac tissue volume compared to WT littermates.

Conclusion: We here demonstrate that Na_V 1.5 is involved in cardiac electrical function from E10.5 onwards. Scn5a-1798insD^-/- embryos displayed cardiac structural abnormalities at E9.5, indicating that Na_V 1.5 dysfunction impacts on embryonic cardiac development in a non-electrogenic manner. These findings are potentially relevant for understanding structural defects observed in relation to Na_V 1.5 dysfunction.

Keywords: SCN5A; cardiac morphology; development; embryonic lethality; non-electrogenic; sodium current.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Mice
Myocytes, Cardiac* / metabolism
NAV1.5 Voltage-Gated Sodium Channel* / genetics
Sodium / metabolism

Substances

NAV1.5 Voltage-Gated Sodium Channel
Sodium