Novel mutations in the resistome of a new sequence type (ST262) of clarithromycin resistant Mycobacterium abscessus subsp. massiliense

Maiara Dos Santos Carneiro; Daiana de Lima-Morales; Marina Niada Crispim; Luciana de Souza Nunes; Afonso Luis Barth

doi:10.1016/j.jgar.2020.04.030

Novel mutations in the resistome of a new sequence type (ST262) of clarithromycin resistant Mycobacterium abscessus subsp. massiliense

J Glob Antimicrob Resist. 2020 Jun:21:294-295. doi: 10.1016/j.jgar.2020.04.030. Epub 2020 May 6.

Authors

Maiara Dos Santos Carneiro¹, Daiana de Lima-Morales², Marina Niada Crispim², Luciana de Souza Nunes³, Afonso Luis Barth⁴

Affiliations

¹ Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
² Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
³ Universidade Federal do Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
⁴ Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil. Electronic address: albarth@hcpa.edu.br.

PMID: 32387639
DOI: 10.1016/j.jgar.2020.04.030

Abstract

Objectives: The aim of this study was to evaluate the relationship between antimicrobial susceptibility and genotype of a Mycobacterium abscessus subsp. massiliense isolate obtained from the respiratory tract of a patient in southern Brazil.

Methods: The isolate (named Myco1POA) was submitted to whole-genome sequencing using an Illumina MiSeq platform. Data were analysed using Trim Galore!, SPAdes Genome Assembler, Geneious and BioEdit software. Multilocus sequence typing (MLST) was performed by in silico analysis of seven housekeeping genes according to the Institut Pasteur database. The antimicrobial susceptibility profile was determined by broth microdilution according to Clinical and Laboratory Standards Institute (CLSI) guidelines.

Results: Several mutations in genes related to antimicrobial resistance were identified in Myco1POA. MLST indicated that the isolate belonged to a novel sequence type (ST), which was designated ST262. Phenotypic susceptibility and genotypic findings were concordant, except for clarithromycin [erm(41) and rrl genes].

Conclusion: Here we describe the genome sequence of M. abscessus subsp. massiliense Myco1POA identified as a novel sequence type (ST262) and indicate possible new gene mutations leading to clarithromycin resistance.

Keywords: Genome sequence; Mycobacterium abscessus subsp. massiliense; Novel sequence type; Resistome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Brazil
Clarithromycin* / pharmacology
Humans
Microbial Sensitivity Tests
Multilocus Sequence Typing
Mutation
Mycobacterium abscessus* / genetics

Substances

Anti-Bacterial Agents
Clarithromycin