[The effect of acetoacetate on 3-hydroxybutyrate oxidation by rat liver mitochondria]

Ukr Biokhim Zh (1978). 1988 Nov-Dec;60(6):79-82.
[Article in Russian]

Abstract

Effect of acetoacetate on 3-hydroxybutyrate oxidation by rat liver mitochondria is described. State 3 respiration is inhibited by acetoacetate, while state 4 respiration is not inhibited, though cytochrome c reduction was decreased. Acetoacetate is also non-competitive inhibitor of 3-hydroxybutyrateoxidase and 3-hydroxybutyrate dehydrogenase activity in frozen-thawed mitochondria. The results are discussed in terms of the thermodynamic hypothesis and control strength method.

Publication types

  • English Abstract

MeSH terms

  • 3-Hydroxybutyric Acid
  • Acetoacetates / pharmacology*
  • Animals
  • Hydroxybutyrate Dehydrogenase / antagonists & inhibitors
  • Hydroxybutyrates / metabolism*
  • In Vitro Techniques
  • Kinetics
  • Mitochondria, Liver / enzymology
  • Mitochondria, Liver / metabolism*
  • Oxidation-Reduction
  • Oxidative Phosphorylation / drug effects
  • Rats

Substances

  • Acetoacetates
  • Hydroxybutyrates
  • Hydroxybutyrate Dehydrogenase
  • 3-Hydroxybutyric Acid