Genetic algorithm-based personalized models of human cardiac action potential

PLoS One. 2020 May 11;15(5):e0231695. doi: 10.1371/journal.pone.0231695. eCollection 2020.


We present a novel modification of genetic algorithm (GA) which determines personalized parameters of cardiomyocyte electrophysiology model based on set of experimental human action potential (AP) recorded at different heart rates. In order to find the steady state solution, the optimized algorithm performs simultaneous search in the parametric and slow variables spaces. We demonstrate that several GA modifications are required for effective convergence. Firstly, we used Cauchy mutation along a random direction in the parametric space. Secondly, relatively large number of elite organisms (6-10% of the population passed on to new generation) was required for effective convergence. Test runs with synthetic AP as input data indicate that algorithm error is low for high amplitude ionic currents (1.6±1.6% for IKr, 3.2±3.5% for IK1, 3.9±3.5% for INa, 8.2±6.3% for ICaL). Experimental signal-to-noise ratio above 28 dB was required for high quality GA performance. GA was validated against optical mapping recordings of human ventricular AP and mRNA expression profile of donor hearts. In particular, GA output parameters were rescaled proportionally to mRNA levels ratio between patients. We have demonstrated that mRNA-based models predict the AP waveform dependence on heart rate with high precision. The latter also provides a novel technique of model personalization that makes it possible to map gene expression profile to cardiac function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials* / genetics
  • Action Potentials* / physiology
  • Gene Expression
  • Heart / physiology*
  • Heart Transplantation
  • Heart Ventricles / metabolism
  • Humans
  • Models, Biological
  • Myocytes, Cardiac / physiology*
  • Patch-Clamp Techniques
  • RNA-Seq
  • Tissue Donors

Associated data

  • Dryad/10.5061/dryad.stqjq2c0

Grant support

The research was supported by Russian Foundation for Basic Research ( grants 18-07-01480 (to RS and DS), 19-29-04111 (to RS), 18-00-01524 (to RS) and Leducq Foundation ( project RHYTHM (to IE and KA). RNA-based model development study was supported by Russian Scientific Foundation ( grant 18-71-10058 (to AP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.