Process for an efficient lentiviral cell transduction

Biol Methods Protoc. 2020 Feb 10;5(1):bpaa005. doi: 10.1093/biomethods/bpaa005. eCollection 2020.


The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases in transduction efficiency in various cell lines in comparison to several other procedures.

Keywords: CRISPR-Cas; cell transduction; lentivirus; shRNA.