Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning

Int J Mol Sci. 2020 May 9;21(9):3359. doi: 10.3390/ijms21093359.


Swiprosin-1 (EFhD2) is a molecule that triggers structural adaptation of isolated adult rat cardiomyocytes to cell culture conditions by initiating a process known as cell spreading. This process mimics central aspects of cardiac remodeling, as it occurs subsequent to myocardial infarction. However, expression of swiprosin-1 in cardiac tissue and its regulation in vivo has not yet been addressed. The expression of swiprosin-1 was analyzed in mice, rat, and pig hearts undergoing myocardial infarction or ischemia/reperfusion with or without cardiac protection by ischemic pre- and postconditioning. In mouse hearts, swiprosin-1 protein expression was increased after 4 and 7 days in myocardial infarct areas specifically in cardiomyocytes as verified by immunoblotting and histology. In rat hearts, swiprosin-1 mRNA expression was induced within 7 days after ischemia/reperfusion but this induction was abrogated by conditioning. As in cultured cardiomyocytes, the expression of swiprosin-1 was associated with a coinduction of arrestin-2, suggesting a common mechanism of regulation. Rno-miR-32-3p and rno-miR-34c-3p were associated with the regulation pattern of both molecules. Moreover, induction of swiprosin-1 and ssc-miR-34c was also confirmed in the infarct zone of pigs. In summary, our data show that up-regulation of swiprosin-1 appears in the postischemic heart during cardiac remodeling and repair in different species.

Keywords: cardiac protection; cardiac regeneration; miR-34.

MeSH terms

  • Animals
  • Atrial Remodeling / genetics*
  • Atrial Remodeling / physiology
  • Calcium-Binding Proteins / biosynthesis*
  • Calcium-Binding Proteins / genetics
  • Cells, Cultured
  • Gene Expression Regulation*
  • Ischemic Preconditioning, Myocardial*
  • Mice
  • MicroRNAs / biosynthesis
  • MicroRNAs / genetics
  • Microfilament Proteins / biosynthesis*
  • Microfilament Proteins / genetics
  • Myocardial Infarction / genetics*
  • Myocardial Infarction / metabolism
  • Myocytes, Cardiac / metabolism
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Reperfusion Injury / genetics*
  • Reperfusion Injury / metabolism
  • Swine
  • Ventricular Remodeling / genetics*
  • Ventricular Remodeling / physiology
  • beta-Arrestin 1 / biosynthesis
  • beta-Arrestin 1 / genetics


  • Calcium-Binding Proteins
  • EFHD2 protein, mouse
  • Efhd2 protein, rat
  • MIRN34 microRNA, rat
  • MicroRNAs
  • Microfilament Proteins
  • RNA, Messenger
  • beta-Arrestin 1