A serological assay to detect SARS-CoV-2 seroconversion in humans

Nat Med. 2020 Jul;26(7):1033-1036. doi: 10.1038/s41591-020-0913-5. Epub 2020 May 12.

Abstract

Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.

Publication types

  • Observational Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Betacoronavirus / immunology*
  • Betacoronavirus / pathogenicity
  • Case-Control Studies
  • Clinical Laboratory Techniques / methods*
  • Coronavirus Infections / blood
  • Coronavirus Infections / diagnosis*
  • Coronavirus Infections / immunology*
  • Coronavirus Infections / therapy
  • Coronavirus Infections / virology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunization, Passive
  • Longitudinal Studies
  • Middle Aged
  • Neutralization Tests
  • Pandemics
  • Pneumonia, Viral / diagnosis*
  • Pneumonia, Viral / immunology*
  • Pneumonia, Viral / therapy
  • Pneumonia, Viral / virology
  • Seroconversion*
  • Young Adult

Supplementary concepts

  • COVID-19
  • COVID-19 diagnostic testing
  • COVID-19 serotherapy
  • severe acute respiratory syndrome coronavirus 2