Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse

Rui Tian; He Zou; Lu-Fei Wang; Mei-Jiao Song; Lu Liu; Hui Zhang

doi:10.18240/ijo.2020.04.02

Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse

Int J Ophthalmol. 2020 Apr 18;13(4):535-544. doi: 10.18240/ijo.2020.04.02. eCollection 2020.

Authors

Rui Tian¹, He Zou¹, Lu-Fei Wang¹, Mei-Jiao Song¹, Lu Liu¹, Hui Zhang¹

Affiliation

¹ Department of Ophthalmology, the Second Hospital of Jilin University, Changchun 130000, Jilin Province, China.

Abstract

Aim: To explore the mRNA and pathways related to retinoblastoma (RB) genesis and development.

Methods: Microarray datasets GSE29683 (human) and GSE29685 (mouse) were downloaded from NCBI GEO database. Homologous genes between the two species were identified using WGCNA, followed by protein-protein interaction (PPI) network construction and gene enrichment analysis. Disease-related miRNAs and pathways were retrieved from miR2Disease database and Comparative Toxicogenomics Database (CTD), respectively.

Results: A total of 352 homologous genes were identified. Two pathways including "cell cycle" and "pathway in cancer" in CTD and enrichment analysis were identified and seven miRNAs (including hsa-miR-373, hsa-miR-34a, hsa-miR-129, hsa-miR-494, hsa-miR-503, hsa-let-7 and hsa-miR-518c) were associated with RB. miRNAs modulate "cell cycle" and "pathway in cancer" pathways via regulating 13 genes (including CCND1, CDC25C, E2F2, CDKN2D and TGFB2).

Conclusion: These results suggest that these miRNAs play crucial roles in RB genesis through "cell cycle" and "pathway in cancer" pathways by regulating their targets including CCND1, CDC25C, E2F2 and CDKN2D.

Keywords: Kyoto Encyclopedia of Genes and Genomes pathway; microRNA; retinoblastoma; weighted gene co-expression network analysis.

International Journal of Ophthalmology Press.