Cannabidiol on 5-FU-induced oral mucositis in mice

Oral Dis. 2020 Oct;26(7):1483-1493. doi: 10.1111/odi.13413. Epub 2020 Jun 1.


Purpose: The aim of this study was to evaluate the clinical, histological, hematological, and oxidative stress effects of cannabidiol (CBD) in mice with induced oral mucositis.

Methods: We used 90 mice of the CF-1 strain in which oral mucositis was induced using a protocol with 5-fluorouracil (5-FU) chemotherapy. The animals were divided randomly into 10 study groups. Three groups were treated with different doses of CBD (3, 10, and 30 mg/kg), while 2 were control groups (positive control: 5-FU + mechanical trauma + placebo; and negative control: mechanical trauma + placebo), and 2 experimental times were studied (4 and 7 days). All treatments were by intraperitoneal administration.

Results: In the clinical evaluation, the groups treated with CBD showed less severity of oral lesions compared with the positive control at both experimental times. The intensity of the inflammatory response was also lower in the groups treated with this drug, but there was no statistically significant difference when compared with the positive control. With regard to erythrocyte, leukocyte, and platelet counts and anti-oxidant enzyme activity, the groups treated with CBD showed better results, but only some of these variables showed statistically significant differences.

Conclusions: CBD seems to exert an anti-inflammatory and anti-oxidant activity favoring a faster resolution of oral mucositis in this animal model.

Keywords: 5-FU chemotherapy; CAT; GSH; anti-oxidant enzymes; cannabidiol; erythrocyte count; inflammatory score; mice; mucositis classification; oral mucositis; platelet count; white blood cell count.

MeSH terms

  • Animals
  • Cannabidiol
  • Disease Models, Animal
  • Fluorouracil / adverse effects
  • Intestinal Mucosa
  • Mice
  • Mucositis*
  • Stomatitis* / chemically induced
  • Stomatitis* / drug therapy


  • Cannabidiol
  • Fluorouracil