2-Epi-anthracimycin, a new cytotoxic agent from the marine-derived actinomycete Streptomyces sp. OPMA00631

J Antibiot (Tokyo). 2020 Aug;73(8):548-553. doi: 10.1038/s41429-020-0309-2. Epub 2020 May 13.

Abstract

A new cytotoxic agent designated as 2-epi-anthracimycin (1) was isolated along with anthracimycin and anthracimycin B (2-demethylanthracimycin) from the culture broth of the marine-derived actinomycete Streptomyces sp. OPMA00631. The structure of 1 was elucidated based on spectroscopic analyses (1D and 2D NMR data and ROESY correlations). Compound 1 exhibited cytotoxicity against Jurkat cells with an IC50 value of 50.5 μM in 20 h. The effect of 1 on the cell cycle distribution of Jurkat cells was investigated. Compound 1 (7.80 μM) increased G1 phase cells from 51.1 to 62.0% and conversely, decreased G2 and M phase cells from 30.7 to 19.3 % in 20 h. At a higher concentration, 1 (250 μM) markedly increased subG1 phase cells (1.9% at 0 h to 16.5% at 20 h), while the proportion of G1 phase cells was maintained (62.3%). These results suggest that 1 exhibits cytotoxicity against Jurkat cells by arresting the cell cycle at the G1 phase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / chemistry*
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Aquatic Organisms / chemistry*
  • Cell Cycle / drug effects
  • Cell Line, Tumor
  • Cytotoxins / chemistry*
  • Cytotoxins / pharmacology*
  • Humans
  • Jurkat Cells
  • Polyketides / chemistry*
  • Polyketides / pharmacology*
  • Streptomyces / chemistry*

Substances

  • Antineoplastic Agents
  • Cytotoxins
  • Polyketides
  • anthracimycin