Abstract
A highly sensitive and specific Hg-whole-cell biosensor was developed from a non-selective variant of the Au sensor GolS and its regulatory pathway. The performance of this analytical tool was validated under laboratory and field-like conditions. This biosensor can be easily applied in cost-effective and portable semiquantitative devices to report Hg contamination in water.
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Biosensing Techniques / methods*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Fresh Water / analysis
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Gold / chemistry*
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Ligands
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Limit of Detection
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Mercury / analysis*
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Salmonella / metabolism*
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Spectrometry, Fluorescence
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Water Pollutants, Chemical / analysis*
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Ligands
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MerR protein, Bacteria
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Water Pollutants, Chemical
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Green Fluorescent Proteins
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Gold
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Mercury