Lipids are an essential macromolecule used for diverse functions including use for structural components of membranes, energy storage, and signaling molecules. The regulation of cellular lipid stores is critical for maintaining organismal metabolic homeostasis. Lipid homeostasis can decline with age, which can lead to poor health outcomes and accelerate the progression of disease states. C. elegans represents an excellent model to study age-related decline in lipid homeostasis due to its short lifespan and remarkably well-conserved metabolic pathways. Due to their ease of use and similarities, there have been numerous developments in methodologies to study intracellular lipid abundance and tissue distribution in C. elegans.
Keywords: Abundance; Aging; C. elegans; Distribution; Fat; Germline; Intestine; Lipids; Nile red; Oil red O.