Wet-adhesive, haemostatic and antimicrobial bilayered composite nanosheets for sealing and healing soft-tissue bleeding wounds

Chengkai Xuan; Lijin Hao; Xuemin Liu; Ye Zhu; Huishang Yang; Yipeng Ren; Lin Wang; Toshinori Fujie; Hongkai Wu; Yunhua Chen; Xuetao Shi; Chuanbin Mao

doi:10.1016/j.biomaterials.2020.120018

Wet-adhesive, haemostatic and antimicrobial bilayered composite nanosheets for sealing and healing soft-tissue bleeding wounds

Biomaterials. 2020 Sep:252:120018. doi: 10.1016/j.biomaterials.2020.120018. Epub 2020 Apr 15.

Authors

Chengkai Xuan¹, Lijin Hao¹, Xuemin Liu¹, Ye Zhu², Huishang Yang³, Yipeng Ren⁴, Lin Wang⁵, Toshinori Fujie⁶, Hongkai Wu⁷, Yunhua Chen⁸, Xuetao Shi⁹, Chuanbin Mao¹⁰

Affiliations

¹ School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China.
² Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, 101 Stephenson Parkway, Norman, OK, 73019-5300, USA.
³ Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005, Guangzhou, PR China.
⁴ Department of Stomatology, Chinese PLA General Hospital, Beijing, 100853, PR China.
⁵ Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, 510006, PR China.
⁶ Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, B-50, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi-shi, Saitama, 332-0012, Japan.
⁷ Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PR China.
⁸ School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China. Electronic address: msyhchen@scut.edu.cn.
⁹ School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005, Guangzhou, PR China. Electronic address: mrshixuetao@gmail.com.
¹⁰ Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, 101 Stephenson Parkway, Norman, OK, 73019-5300, USA; School of Materials Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China. Electronic address: cbmao@ou.edu.

PMID: 32417650
DOI: 10.1016/j.biomaterials.2020.120018

Abstract

Healing soft-tissue wounds with an irregular, complicated topography in a bleeding environment demands the development of a dressing that is wet-adhesive, haemostatic, and antibacterial. To meet this unmet demand, we designed a flexible nanosheet (~77 nm thick) made of two layers, one is the antibacterial and haemostatic gelatin modified with dopamine (DA) and antimicrobial peptide (AMP) and mixed with Ca²⁺ ions as coagulation factors, and another is the mechanically strong polycaprolactone (PCL). This flexible nanosheet exhibited robust mechanical strength, continuous and effective adhesion to a topographically irregular tissue surface under a wet condition, and a high platelet adhesion capacity. Moreover, the nanosheet presented a significantly reduced clotting time of 4 min and a high bactericidal rate of nearly 100%. An in vivo evaluation of the nanosheet using both murine dorsal skin and liver models further revealed that the nanosheet could successfully seal and heal the wounds in a bleeding environment, efficiently control haemorrhaging, and exert an excellent antibacterial effect in two weeks. Our work suggests that this nanosheet holds great promise in healing the bleeding soft-tissue wounds for treating acute trauma.

Keywords: Complicated topography; Haemostatic; Nanosheet; Wet adhesive.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adhesives*
Animals
Anti-Bacterial Agents / therapeutic use
Bandages
Hemostatics*
Mice
Wound Healing

Substances

Adhesives
Anti-Bacterial Agents
Hemostatics