Radioimmunoassay of total and free corticosterone in rat plasma: measurement of the effect of different doses of corticosterone

Steroids. 1988 May-Jun;51(5-6):609-22. doi: 10.1016/0039-128x(88)90056-6.


A radioimmunological method was developed for determining total and free corticosterone in rat plasma. This method was used to determine the dose-response curve of corticosterone and to measure the elimination and study the half-lives of total and free corticosterone in rat plasma with a dose of 5 mg/kg. The elimination with a dose of 5 mg/kg, when drawn on the half-logarithmic scale, formed a straight line. The half-lives for total and free corticosterone were 25 and 15 min, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Corticosterone / blood*
  • Corticosterone / pharmacokinetics
  • Cross Reactions
  • Dose-Response Relationship, Drug
  • Half-Life
  • Male
  • Radioimmunoassay
  • Rats
  • Rats, Inbred Strains


  • Corticosterone