Prevalence and Characterization of Plasmid-Mediated Quinolone Resistance Determinants qnr and aac( 6')- Ib- cr in Ciprofloxacin-Resistant Escherichia coli Isolates from Commercial Layer in Korea

J Microbiol Biotechnol. 2020 Aug 28;30(8):1180-1183. doi: 10.4014/jmb.2003.03058.

Abstract

The prevalence and characterization of plasmid-mediated quinolone resistance (PMQR) determinants in ciprofloxacin-resistant Escherichia coli isolated from a Korean commercial layer farm were studied. A total of 45 ciprofloxacin-resistant E. coli isolates were recovered and all isolates were multidrug-resistant. Eight isolates have the PMQR genes aac(6')-Ib-cr, qnrS1, and qnrB4, and seven isolates exhibited double amino acid exchange at both gyrA and parC, and have high fluoroquinolone minimum inhibitory concentrations. Five transconjugants demonstrated transferability of PMQR and β-lactamase genes and similar antimicrobial resistance. Because PMQR genes in isolates from commercial layer chickens could enter the food supply and directly affect humans, control of ciprofloxacin resistance is needed.

Keywords: Antimicrobial resistance; Escherichia coli; commercial layer chicken; fluoroquinolones; plasmid-mediated quinolone resistance.