Monitoring protein ubiquitination and SUMOylation in real-time by NMR

Chem Commun (Camb). 2020 Jun 18;56(49):6735-6738. doi: 10.1039/d0cc02252g.


The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.

MeSH terms

  • Humans
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular*
  • Sumoylation*
  • Time Factors
  • Ubiquitin / chemistry
  • Ubiquitin / metabolism*
  • Ubiquitinated Proteins / analysis*
  • Ubiquitinated Proteins / chemistry*
  • Ubiquitination*


  • Ubiquitin
  • Ubiquitinated Proteins