A Mouse Nasopharyngeal Colonization Model for Group A Streptococcus

Methods Mol Biol. 2020;2136:303-308. doi: 10.1007/978-1-0716-0467-0_23.

Abstract

Group A Streptococcus (GAS) often exists as an asymptomatic colonizer of the upper respiratory tract in humans. Unsurprisingly, a high proportion of symptomatic infections caused by GAS include pharyngitis. While not usually life-threatening, these infections cause significant morbidity and economic burden/loss of productivity, and can have downstream life-threatening autoimmune consequences. Modeling asymptomatic colonization in animals is, therefore, a useful tool to dissect host-bacteria interactions and to evaluate efficacy of vaccines aimed at reducing the burden of carriage. Here we describe a mouse model of nasopharyngeal colonization using nasal challenge of susceptible mice and the evaluation of subsequent bacterial burden.

Keywords: FVB/n mouse; Mouse model; Nasopharyngeal colonization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / immunology
  • Disease Models, Animal
  • Female
  • Humans
  • Male
  • Mice
  • Mice, Inbred Strains
  • Nasopharyngeal Diseases / microbiology
  • Nasopharynx / microbiology*
  • Streptococcal Infections / immunology*
  • Streptococcal Infections / metabolism
  • Streptococcus pyogenes / immunology*
  • Streptococcus pyogenes / pathogenicity

Substances

  • Antigens, Bacterial