Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium

Tomoyo Sawada; Kynon J M Benjamin; Anna C Brandtjen; Ethan Tietze; Samuel J Allen; Apuã C M Paquola; Joel E Kleinman; Thomas M Hyde; Jennifer A Erwin

doi:10.1016/j.scr.2020.101806

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium

Stem Cell Res. 2020 Jul:46:101806. doi: 10.1016/j.scr.2020.101806. Epub 2020 Apr 20.

Authors

Tomoyo Sawada¹, Kynon J M Benjamin², Anna C Brandtjen³, Ethan Tietze³, Samuel J Allen³, Apuã C M Paquola⁴, Joel E Kleinman⁵, Thomas M Hyde², Jennifer A Erwin⁶

Affiliations

¹ Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA. Electronic address: Tomoyo.Sawada@libd.org.
² Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA; Department of Psychiatry & Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
³ Lieber Institute for Brain Development, Baltimore, Maryland, USA.
⁴ Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
⁵ Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Psychiatry & Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
⁶ Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland, USA. Electronic address: Jennifer.Erwin@libd.org.

Abstract

In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Brain
Cell Differentiation
Cell Line
Fibroblasts
Genotype
Humans
Induced Pluripotent Stem Cells*
Kruppel-Like Factor 4
Transcriptome

Abstract

Publication types

MeSH terms

Grants and funding