An Immunofluorescent Method Using Crithidia Luciliae to Detect Antibodies to Double-Stranded DNA

Arthritis Rheum. 1977 Apr;20(3):811-4. doi: 10.1002/art.1780200308.

Abstract

The immunochemical specificity of the immunofluorescent Crithidia luciliae method for detection of antibodies to double-stranded DNA (dsDNA) was confirmed by demonstrating abolition of staining by DNase digestion and by absorption with dsDNA. This method was less sensitive than a Millipore filter method for detecting antibodies to DNA. It was positive only in subjects with systemic lupus erythematosus or drug-induced antinuclear factors. This technique appears suitable for study of the immunochemical characteristics fo antibodies to dsDNA.

MeSH terms

  • Antibodies, Antinuclear / analysis*
  • DNA / immunology*
  • Fluorescent Antibody Technique*
  • Humans
  • Lupus Erythematosus, Systemic / immunology
  • Micropore Filters
  • Trypanosoma / immunology

Substances

  • Antibodies, Antinuclear
  • DNA