The pseudorabies virus R2 non-neuroinvasive vaccine: A proof-of-concept study in pigs

Vaccine. 2020 Jun 15;38(29):4524-4528. doi: 10.1016/j.vaccine.2020.05.002. Epub 2020 May 22.


Neurotropic alpha-herpesviruses that infect mammals establish life-long latent infections in the peripheral nervous system after initial infection of exposed mucosal tissues. The neuroinvasive properties can lead to severe complications both with clinical and veterinary alpha-herpesviruses, and vaccines are often unavailable or provide limited protection. Here we assess the properties and efficacy of an R2 vaccine derived from the alpha-herpesvirus, pseudorabies virus (PRV), in pigs. We demonstrate that the PRV R2 vaccine does not invade the porcine peripheral nervous system within the limits of detection. Furthermore, after a single intranasal vaccination, R2 conferred protection to pigs subsequently challenged with a virulent PRV field strain (NIA-3). These findings support that the R2 vaccine design is non-neuroinvasive and is an effective vaccine in the context of a natural host.

MeSH terms

  • Animals
  • Antibodies, Viral
  • Herpesvirus 1, Suid*
  • Pseudorabies Vaccines
  • Pseudorabies* / prevention & control
  • Swine
  • Swine Diseases* / prevention & control
  • Vaccines*
  • Viral Vaccines*


  • Antibodies, Viral
  • Pseudorabies Vaccines
  • Vaccines
  • Viral Vaccines