Response surface optimization of phenolic compounds from jabuticaba (Myrciaria cauliflora [Mart.] O.Berg) seeds: Antioxidant, antimicrobial, antihyperglycemic, antihypertensive and cytotoxic assessments

Food Chem Toxicol. 2020 Aug:142:111439. doi: 10.1016/j.fct.2020.111439. Epub 2020 May 22.


The aim of this study was to evaluate the effects of different solvents and maximize the extraction of bioactive compounds from jabuticaba (Myrciaria cauliflora) seeds. In general, the solvent system composed of water and propanone (52:48 v/v) modified the extract polarity and increased extraction yield of bioactive compounds. The optimized extract presented antioxidant capacity measured by different chemical and biological assays. The optimized extract exerted antiproliferative and cytotoxic effects against A549 and HCT8 cells, antimicrobial and antihemolytic effects, inhibited α-amylase/α-glucosidase activities and presented in vitro antihypertensive effect. Nonetheless, the optimized extract showed no cytotoxicity in a human cell model (IMR90). Vescalagin, castalagin and ellagic acid were the major phenolic compounds in the optimized extract. Our results show that jabuticaba seed may be a potential ingredient for the development of potentially functional foods.

Keywords: Bioactivity; Ellagitannins; Functional foods; Natural resources; Response surface methodology.

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / pharmacology
  • Anti-Infective Agents / pharmacology
  • Antihypertensive Agents / pharmacology
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Antioxidants / pharmacology
  • Cell Line, Tumor
  • Humans
  • Hypoglycemic Agents / pharmacology
  • Lipid Peroxidation / drug effects
  • Microbial Sensitivity Tests
  • Myrtaceae / embryology*
  • Phenols / analysis*
  • Plant Extracts / pharmacology*
  • Seeds / chemistry*


  • Angiotensin-Converting Enzyme Inhibitors
  • Anti-Infective Agents
  • Antihypertensive Agents
  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Hypoglycemic Agents
  • Phenols
  • Plant Extracts