jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths

Nat Methods. 2020 Jul;17(7):694-697. doi: 10.1038/s41592-020-0835-7. Epub 2020 May 25.


Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Calcium / analysis*
  • Drosophila
  • Female
  • Fluorescent Dyes / chemistry*
  • Lasers
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Molecular Imaging
  • Somatosensory Cortex / chemistry


  • Fluorescent Dyes
  • Calcium