Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS/JNK/NF-κB/MMP-1 pathway

Lasers Med Sci. 2020 Oct;35(8):1831-1839. doi: 10.1007/s10103-020-03040-z. Epub 2020 May 26.


Photobiomodulation (PBM) has been shown to improve wound healing by promoting mesenchymal stem cell migration and proliferation. However, it remains unknown whether an 808-nm diode laser can influence human gingival mesenchymal stem cells (HGMSCs), and which dose this works well. In the present study, it was found that PBM could promote the migration of HGMSCs but not the proliferation. Furthermore, PBM could activate mitochondrial ROS, which could elevate the phosphorylation levels of JNK and IKB in HGMSCs, and further activate NF-κB as the nuclear translocation of p65 is elevated. Taken together, these present results indicate that PBM might promote cell migration via the ROS/JNK/NF-κB pathway.

Keywords: JNK; Migration; Mitochondria; NF-κB; PBM; ROS.

MeSH terms

  • Cell Movement / radiation effects*
  • Gingiva / cytology
  • Gingiva / physiology*
  • Gingiva / radiation effects*
  • Humans
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Lasers, Semiconductor / therapeutic use*
  • MAP Kinase Signaling System / radiation effects*
  • Matrix Metalloproteinase 1 / metabolism
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / radiation effects
  • Mitochondria / metabolism
  • Mitochondria / radiation effects
  • NF-kappa B / metabolism
  • Phosphorylation / radiation effects
  • Reactive Oxygen Species / metabolism
  • Wound Healing / radiation effects*


  • NF-kappa B
  • Reactive Oxygen Species
  • JNK Mitogen-Activated Protein Kinases
  • Matrix Metalloproteinase 1