Browning of the subcutaneous adipocytes in diet-induced obese mouse submitted to intermittent fasting

Thatiany de Souza Marinho; Fernanda Ornellas; Marcia Barbosa Aguila; Carlos Alberto Mandarim-de-Lacerda

doi:10.1016/j.mce.2020.110872

Browning of the subcutaneous adipocytes in diet-induced obese mouse submitted to intermittent fasting

Mol Cell Endocrinol. 2020 Aug 1:513:110872. doi: 10.1016/j.mce.2020.110872. Epub 2020 May 23.

Authors

Thatiany de Souza Marinho¹, Fernanda Ornellas¹, Marcia Barbosa Aguila¹, Carlos Alberto Mandarim-de-Lacerda²

Affiliations

¹ Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology, The University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
² Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology, The University of the State of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: mandarim@uerj.br.

PMID: 32454192
DOI: 10.1016/j.mce.2020.110872

Abstract

Purpose: We studied subcutaneous white adipose tissue (sWAT) of obese mice submitted to intermittent fasting (IF).

Methods: Twelve-week-old C57BL/6 male mice received the diets Control (C) or high-fat (HF) for eight weeks (n = 20/each). Then, part of each group performed IF (24 h feeding/24 h fasting) for four weeks: C, C-IF, HF, and HF-IF (n = 10/each).

Results: Food intake did not show a difference in feeding and fasting days, but HF groups had a high energy intake. IF led to multilocular adipocytes in sWAT (browning), and improved respiratory quotient on the fed day. IF decreased gene expression of Leptin, but increased Adiponectin, β3ar (beta3 adrenoreceptor), and Ucp1 (uncoupling protein). IF enhanced immunostaining of Caspase 3, Pcna (proliferating cell nuclear antigen), and UCP1 in sWAT. IF attenuated pro-inflammatory markers and pro-apoptotic markers in sWAT.

Conclusions: IF in obese mice led to browning in sWAT adipocytes, enhanced thermogenesis, an improved adipose tissue pro-inflammatory profile.

Keywords: Cell proliferation; Inflammation; Thermogenesis; Uncoupling protein 1; beta3 adrenoreceptor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes, Brown / physiology*
Adipocytes, White / physiology*
Animals
Cell Transdifferentiation
Diet, High-Fat
Energy Intake / physiology
Fasting / physiology*
Male
Mice
Mice, Inbred C57BL
Obesity / etiology
Obesity / physiopathology*
Subcutaneous Fat / cytology*
Subcutaneous Fat / physiology
Thermogenesis / physiology