Quantification of Galantamine in Sternbergia Species by High Performance Liquid Chromatography

Özlem Bahadır Acikara; Betül Sever Yilmaz; Dilhun Yazgan; Gülçin Saltan Işcan

doi:10.4274/tjps.95967

Quantification of Galantamine in Sternbergia Species by High Performance Liquid Chromatography

Turk J Pharm Sci. 2019 Mar;16(1):32-36. doi: 10.4274/tjps.95967. Epub 2018 Dec 31.

Authors

Özlem Bahadır Acikara¹, Betül Sever Yilmaz¹, Dilhun Yazgan¹, Gülçin Saltan Işcan¹

Affiliation

¹ Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey.

Abstract

Objectives: This study describes the qualitative and quantitative analysis of galantamine in Sternbergia species growing in Turkey.

Materials and methods: Galantamine was isolated from Sternbergia fischeriana bulbs and the structure of the compound elucidated by spectroscopic methods. The qualitative and quantitative analysis of galantamine was investigated in Sternbergia lutea subsp. lutea, S. lutea subsp. sicula, Sternbergia candida, S. fischeriana, and Sternbergia clusiana using a specially developed and validated high performance liquid chromatography (HPLC) method.

Results: S. lutea subsp. sicula had the highest content of galantamine, i.e., 0.0165±0.0002 g/100 g. The limits of detection and quantification were 7.5 μg and 25 μg, respectively.

Conclusion: Isolation of galantamine from S. fischeriana growing in Turkey is reported for the first time. An HPLC method was developed for identification and quantification of galantamine in Sternbergia species.

Keywords: Galantamine; HPLC; Sternbergia spp.