Detergent-free Extraction, Reconstitution and Characterization of Membrane-Anchored cytochrome-b5 in Native Lipids

Chem Commun (Camb). 2020 Jun 18;56(48):6511-6514. doi: 10.1039/d0cc01737j. Epub 2020 May 28.

Abstract

Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using nanodiscs for high-resolution studies and revealed the types of native lipids associated with the protein.