Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids

Chem Commun (Camb). 2020 Jun 18;56(48):6511-6514. doi: 10.1039/d0cc01737j. Epub 2020 May 28.

Abstract

Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using nanodiscs for high-resolution studies and revealed the types of native lipids associated with the protein.

MeSH terms

  • Animals
  • Chromatography, Gel
  • Cytochromes b5 / chemistry
  • Cytochromes b5 / isolation & purification
  • Cytochromes b5 / metabolism*
  • Escherichia coli / metabolism
  • Magnetic Resonance Spectroscopy
  • Membrane Lipids / chemistry*
  • Membrane Lipids / metabolism
  • Nanostructures / chemistry
  • Polymers / chemistry
  • Rabbits

Substances

  • Membrane Lipids
  • Polymers
  • Cytochromes b5