Apoplastic Fluid Preparation from Arabidopsis thaliana Leaves Upon Interaction with a Nonadapted Powdery Mildew Pathogen

Methods Mol Biol. 2020;2139:79-88. doi: 10.1007/978-1-0716-0528-8_6.


Proteins in the extracellular space (apoplast) play a crucial role at the interface between plant cells and their proximal environment. Consequently, it is not surprising that plants actively control the apoplastic proteomic profile in response to biotic and abiotic cues. Comparative quantitative proteomics of plant apoplastic fluids is therefore of general interest in plant physiology. We here describe an efficient method to isolate apoplastic fluids from Arabidopsis thaliana leaves inoculated with a nonadapted powdery mildew pathogen.

Keywords: Apoplast; Arabidopsis thaliana; Cell wall; Membrane trafficking; Protein secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / chemistry*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / metabolism
  • Cell Membrane / chemistry
  • Extracellular Space / chemistry*
  • Plant Diseases / microbiology
  • Plant Leaves / chemistry*
  • Plant Leaves / metabolism
  • Proteomics / methods*
  • Stress, Physiological / physiology


  • Arabidopsis Proteins