Complete Genome Sequence of SARS-CoV-2 in a Tiger from a U.S. Zoological Collection

doi:10.1128/MRA.00468-20

. 2020 May 28;9(22):e00468-20.

doi: 10.1128/MRA.00468-20.

Complete Genome Sequence of SARS-CoV-2 in a Tiger from a U.S. Zoological Collection

Leyi Wang^#¹, Patrick K Mitchell^#², Paul P Calle³, Susan L Bartlett³, Denise McAloose³, Mary Lea Killian^#⁴, Fangfeng Yuan⁵, Ying Fang⁵, Laura B Goodman², Richard Fredrickson¹, François Elvinger², Karen Terio⁶, Kerrie Franzen⁴, Tod Stuber⁴, Diego G Diel², Mia Kim Torchetti⁷

Affiliations

¹ Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA.
² Department of Population Medicine and Diagnostic Sciences, Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
³ Wildlife Conservation Society, Zoological Health Program, Bronx Zoo, Bronx, New York, USA.
⁴ National Veterinary Services Laboratories, Veterinary Services, U.S. Department of Agriculture, Ames, Iowa, USA.
⁵ Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA.
⁶ Zoological Pathology Program, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Brookfield, Illinois, USA.
⁷ National Veterinary Services Laboratories, Veterinary Services, U.S. Department of Agriculture, Ames, Iowa, USA mia.kim.torchetti@usda.gov.

^# Contributed equally.

PMID: 32467283
PMCID: PMC7256270
DOI: 10.1128/MRA.00468-20

Complete Genome Sequence of SARS-CoV-2 in a Tiger from a U.S. Zoological Collection

Leyi Wang et al. Microbiol Resour Announc. 2020.

. 2020 May 28;9(22):e00468-20.

doi: 10.1128/MRA.00468-20.

Authors

Affiliations

¹ Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA.
² Department of Population Medicine and Diagnostic Sciences, Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
³ Wildlife Conservation Society, Zoological Health Program, Bronx Zoo, Bronx, New York, USA.
⁴ National Veterinary Services Laboratories, Veterinary Services, U.S. Department of Agriculture, Ames, Iowa, USA.
⁵ Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA.
⁶ Zoological Pathology Program, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Brookfield, Illinois, USA.
⁷ National Veterinary Services Laboratories, Veterinary Services, U.S. Department of Agriculture, Ames, Iowa, USA mia.kim.torchetti@usda.gov.

^# Contributed equally.

PMID: 32467283
PMCID: PMC7256270
DOI: 10.1128/MRA.00468-20

Abstract

This report describes the identification and characterization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a Malayan tiger in a U.S. zoo.

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[1] Zhou P, Yang X-L, Wang X-G, Hu B, Zhang L, Zhang W, Si H-R, Zhu Y, Li B, Huang C-L, Chen H-D, Chen J, Luo Y, Guo H, Jiang R-D, Liu M-Q, Chen Y, Shen X-R, Wang X, Zheng X-S, Zhao K, Chen Q-J, Deng F, Liu L-L, Yan B, Zhan F-X, Wang Y-Y, Xiao G-F, Shi Z-L. 2020. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579:270–273. doi:10.1038/s41586-020-2012-7. - DOI - PMC - PubMed

[2] Zhou P, Yang X-L, Wang X-G, Hu B, Zhang L, Zhang W, Si H-R, Zhu Y, Li B, Huang C-L, Chen H-D, Chen J, Luo Y, Guo H, Jiang R-D, Liu M-Q, Chen Y, Shen X-R, Wang X, Zheng X-S, Zhao K, Chen Q-J, Deng F, Liu L-L, Yan B, Zhan F-X, Wang Y-Y, Xiao G-F, Shi Z-L. 2020. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579:270–273. doi:10.1038/s41586-020-2012-7. - DOI - PMC - PubMed

[3] US Centers for Disease Control and Prevention. 2020. Real-time RT-PCR panel for detection of 2019-nCoV. US Centers for Disease Control and Prevention, Atlanta, GA: https://www.fda.gov/media/134922/download.

[4] US Centers for Disease Control and Prevention. 2020. Real-time RT-PCR panel for detection of 2019-nCoV. US Centers for Disease Control and Prevention, Atlanta, GA: https://www.fda.gov/media/134922/download.

[5] Quick J, Grubaugh ND, Pullan ST, Claro IM, Smith AD, Gangavarapu K, Oliveira G, Robles-Sikisaka R, Rogers TF, Beutler NA, Burton DR, Lewis-Ximenez LL, Goes de Jesus J, Giovanetti M, Hill SC, Black A, Bedford T, Carroll MW, Nunes M, Alcantara LC Jr, Sabino EC, Baylis SA, Faria NR, Loose M, Simpson JT, Pybus OG, Andersen KG, Loman NJ. 2017. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. Nat Protoc 12:1261–1276. doi:10.1038/nprot.2017.066. - DOI - PMC - PubMed

[6] Quick J, Grubaugh ND, Pullan ST, Claro IM, Smith AD, Gangavarapu K, Oliveira G, Robles-Sikisaka R, Rogers TF, Beutler NA, Burton DR, Lewis-Ximenez LL, Goes de Jesus J, Giovanetti M, Hill SC, Black A, Bedford T, Carroll MW, Nunes M, Alcantara LC Jr, Sabino EC, Baylis SA, Faria NR, Loose M, Simpson JT, Pybus OG, Andersen KG, Loman NJ. 2017. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. Nat Protoc 12:1261–1276. doi:10.1038/nprot.2017.066. - DOI - PMC - PubMed

[7] Chrzastek K, Lee DH, Smith D, Sharma P, Suarez DL, Pantin-Jackwood M, Kapczynski DR. 2017. Use of sequence-independent, single-primer-amplification (SISPA) for rapid detection, identification, and characterization of avian RNA viruses. Virology 509:159–166. doi:10.1016/j.virol.2017.06.019. - DOI - PMC - PubMed

[8] Chrzastek K, Lee DH, Smith D, Sharma P, Suarez DL, Pantin-Jackwood M, Kapczynski DR. 2017. Use of sequence-independent, single-primer-amplification (SISPA) for rapid detection, identification, and characterization of avian RNA viruses. Virology 509:159–166. doi:10.1016/j.virol.2017.06.019. - DOI - PMC - PubMed

[9] Wang L, Stuber T, Camp P, Robbe-Austerman S, Zhang Y. 2016. Whole-genome sequencing of porcine epidemic diarrhea virus by Illumina MiSeq platform, p 201–208. In Wang L. (ed), Animal coronaviruses. Springer, New York, NY.

[10] Wang L, Stuber T, Camp P, Robbe-Austerman S, Zhang Y. 2016. Whole-genome sequencing of porcine epidemic diarrhea virus by Illumina MiSeq platform, p 201–208. In Wang L. (ed), Animal coronaviruses. Springer, New York, NY.

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Complete Genome Sequence of SARS-CoV-2 in a Tiger from a U.S. Zoological Collection

Affiliations

Complete Genome Sequence of SARS-CoV-2 in a Tiger from a U.S. Zoological Collection

Authors

Affiliations

Abstract

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References

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