Toolbox for chemically synthesized histone proteins

Curr Opin Chem Biol. 2020 Oct;58:10-19. doi: 10.1016/j.cbpa.2020.04.016. Epub 2020 May 27.


Histone post-translational modifications play significant roles in gene regulation processes. Among many approaches, chemical protein synthesis has been a successful and promising method for the preparation of homogeneous products of site-specifically modified histones for elucidation of their biological significance. In this short review, we describe the recent advances in synthetic toolbox for histone proteins such as thioester precursors, chemical ubiquitination, and one-pot peptide ligation.

Keywords: Chemical protein synthesis; Histone; Native chemical ligation; Post-translational modifications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Chemistry Techniques, Synthetic
  • Esters / chemistry
  • Histones / chemical synthesis*
  • Histones / metabolism*
  • Peptides / chemistry
  • Ubiquitination


  • Esters
  • Histones
  • Peptides