Intra-Articular Route for the System of Molecules 14G1862 from Centella Asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis

Nutrients. 2020 May 31;12(6):1618. doi: 10.3390/nu12061618.


Current pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested the intra-articular pain relieving properties of a system of molecules from a characterized Centella asiatica extract (14G1862) in a rat model of osteoarthritis induced by monoiodoacetate (MIA). 14G1862 (0.2-2 mg mL-1) was intra-articularly (i.a.) injected 7 days after MIA, behavioural and histological evaluations were performed 14, 30 and 60 days after treatments. Moreover, the effect of 14G1862 on nitrate production and iNOS expression in RAW 264.7 macrophages stimulated with LPS was assessed. In vitro, 14G1862 treatment attenuated LPS-induced NO production and iNOS expression in a comparable manner to celecoxib. In vivo, 14G1862 significantly reduced mechanical allodynia and hyperalgesia, spontaneous pain and motor alterations starting on day 14 up to day 60. The efficacy was higher or comparable to that evoked by triamcinolone acetonide (100 μg i.a.) used as reference drug. Histological evaluation highlighted the improvement of several morphological parameters in MIA + 14G1862-treated animals with particularly benefic effects on joint space and fibrin deposition. In conclusion, i.a. treatment with Centella asiatica is a candidate to be a novel effective approach for osteoarthritis therapy.

Keywords: 14G1862; Centella asiatica; MIA; RAW 264.7 cell line; intra-articular treatment; osteoarthritis; pain.

MeSH terms

  • Analgesics / pharmacology
  • Analgesics / therapeutic use*
  • Animals
  • Arthritis, Experimental / chemically induced
  • Arthritis, Experimental / drug therapy
  • Cell Survival / drug effects
  • Centella / chemistry*
  • Disease Models, Animal
  • Hyperalgesia / drug therapy
  • Injections, Intra-Articular / methods*
  • Iodoacetic Acid
  • Macrophages / drug effects
  • Male
  • Mice
  • Nitrates / metabolism
  • Nitric Oxide Synthase Type II / metabolism
  • Osteoarthritis / drug therapy
  • Osteoarthritis, Knee / drug therapy
  • Pain / drug therapy*
  • Pain Management
  • Plant Extracts
  • RAW 264.7 Cells
  • Rats
  • Rats, Sprague-Dawley
  • Triterpenes / pharmacology
  • Triterpenes / therapeutic use*


  • Analgesics
  • Centella asiatica extract
  • Nitrates
  • Plant Extracts
  • Triterpenes
  • Nitric Oxide Synthase Type II
  • Iodoacetic Acid