A novel totivirus isolated from the phytopathogenic fungus Rhodosporidiobolus odoratus strain GZ2017

Arch Virol. 2020 Aug;165(8):1911-1914. doi: 10.1007/s00705-020-04684-6. Epub 2020 Jun 1.

Abstract

To our knowledge, there have been no reports of mycoviruses infecting Rhodosporidiobolus odoratus. Here, we describe the sequence of a novel mycovirus isolated from R. odoratus, which was designated "Rhodosporidiobolus odoratus RNA virus 1" (RoRV1). Sequence analysis revealed that RoRV1 has two discontinuous open reading frames (ORFs), ORF1 and ORF2, potentially encoding a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis based on RdRp sequences clearly placed RoRV1 in the genus Totivirus, family Totiviridae. The fungus also contains two additional, smaller dsRNAs, which might represent RoRV1 satellite RNAs.

MeSH terms

  • Capsid Proteins / genetics
  • Fungi / virology*
  • Genome, Viral / genetics
  • Open Reading Frames / genetics
  • Phylogeny
  • RNA Viruses / genetics*
  • RNA, Double-Stranded / genetics
  • RNA, Viral / genetics
  • RNA-Dependent RNA Polymerase / genetics
  • Sequence Analysis, DNA / methods
  • Totivirus / genetics*
  • Totivirus / isolation & purification*

Substances

  • Capsid Proteins
  • RNA, Double-Stranded
  • RNA, Viral
  • RNA-Dependent RNA Polymerase