Adaptive methodology to determine hydrophobicity of nanomaterials in situ

PLoS One. 2020 Jun 3;15(6):e0233844. doi: 10.1371/journal.pone.0233844. eCollection 2020.

Abstract

The hydrophobicity of nanoparticles (NPs) is a key property determining environmental fate, biological partitioning and toxicity. However, methods to characterize surface hydrophobicity are not uniformly applied to NPs and cannot quantify surface changes in complex environments. Existing methods designed to evaluate the hydrophobicity of bulk solids, chemicals, and proteins have significant limitations when applied to NPs. In this study, we modified and evaluated two methods to determine the hydrophobicity of NPs, hydrophobic interaction chromatography (HIC) and dye adsorption, and compared them to the standard octanol-water partitioning protocol for chemicals. Gold, copper oxide, silica, and amine-functionalized silica NPs were used to evaluate methods based on their applicability to NPs that agglomerate and have surface coatings. The octanol water partitioning and HIC methods both measured Au NPs as hydrophilic, but despite having a small size and stable suspension, NPs could not be fully recovered from the HIC column. For the dye adsorption method, hydrophobic (Rose Bengal) and hydrophilic (Nile Blue) dyes were adsorbed to the NP surface, and linear isotherm parameters were used as a metric for hydrophobicity. CuO was determined to be slightly hydrophilic, while SiO2 was hydrophilic and Ami-SiO2 was hydrophobic. The advantages and limitations of each method are discussed, and the dye adsorption method is recommended as the most suitable for application across broad classes of nanomaterials. The dye assay method was further used to measure changes in the surface hydrophobicity of TiO2 NPs after being suspended in natural water collected from the Alsea Rivers watershed in Oregon. TiO2 NPs adsorbed Rose Bengal when suspended in ultrapure water, but adsorbed Nile Blue after being incubated in natural water samples, demonstrating a shift from hydrophobic to hydrophilic properties on the outer surface. The dye adsorption method can be applied to characterize surface hydrophobicity of NPs and quantify environmental transformations, potentially improving environmental fate models.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adsorption
  • Chromatography / methods*
  • Copper / chemistry
  • Fluorescent Dyes / chemistry*
  • Gold / chemistry
  • Hydrophobic and Hydrophilic Interactions*
  • Nanoparticles / chemistry*
  • Oxazines / chemistry*
  • Rose Bengal / chemistry*
  • Silicon Dioxide / chemistry
  • Spectrometry, Fluorescence / methods*
  • Surface Properties

Substances

  • Fluorescent Dyes
  • Oxazines
  • Rose Bengal
  • Nile Blue
  • Gold
  • Silicon Dioxide
  • Copper
  • cupric oxide

Grants and funding

This work was partially supported by NSF Grant #1762245 awarded to Stacey Harper. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There was no additional external funding received for this study.