Nuclear DNA synthesis in yeast and the effect of irradiation

Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Apr;31(4):365-75. doi: 10.1080/09553007714550431.

Abstract

The synthesis of DNA can be measured in yeast by following the uptake of 5-bromodeoxy-uridine-5'-triphosphate in a mutant that utilizes deoxythymidine-5'-monophosphate; approximately 60 per cent of the DNA is synthesized semi-conservatively before replication stops. Neither ultraviolet light (U.V.), nor ionizing radiation stimulates repair-type synthesis. Based on the ability to detect small amounts of synthesis, it appears that fewer than ten bases are synthesized per pyrimidine dimer removed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Nucleus / metabolism
  • Cell Nucleus / radiation effects
  • Cobalt Radioisotopes
  • DNA / biosynthesis
  • DNA Replication / radiation effects*
  • Gamma Rays
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / radiation effects*
  • Ultraviolet Rays

Substances

  • Cobalt Radioisotopes
  • DNA