Sphingomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench

Abstract

A Gram-stain-negative, short rod-shaped, yellow bacterium (strain LMO-1^T) was isolated from deep-sea sediment of the Mariana Trench, Challenger Deep. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain LMO-1^T belonged to genus Sphingomonas, with the highest sequence similarity to Sphingomonas formosensis CC-Nfb-2^T (96.3 %), followed by Sphingomonas prati W18RD^T (96.1 %), Sphingomonas arantia 6P^T (96.0 %) and Sphingomonas montana W16RD^T (95.9 %). The predominant polar lipids were phosphatidylethanolamine, sphingoglycolipid, phosphatidylglycerol and phosphatidylcholine. The main cellular fatty acids were summed feature 8 (C_{18 : 1} ω7c and/or C_{18 : 1} ω6c), C_{16 : 0} and C_{14 : 0} 2-OH. The major polyamine was sym-homospermidine and the predominant isoprenoid quinone was ubiquinone-10. The genome DNA G+C content of strain LMO-1^T was 69.2 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain LMO-1^T and CC-Nfb-2^T were 75.9 and 20.5 %, respectively. Based on these data, LMO-1^T should be classified as representing a novel species of the genus Sphingomonas, for which the name Sphingomonas profundi sp. nov. is proposed. The type strain is LMO-1^T (=MCCC 1K04066^T=JCM 33666^T).

Keywords: Mariana Trench; Sphingomonas profundi sp. nov.; deep-sea sediment.