GluN2D-mediated excitatory drive onto medial prefrontal cortical PV+ fast-spiking inhibitory interneurons

PLoS One. 2020 Jun 4;15(6):e0233895. doi: 10.1371/journal.pone.0233895. eCollection 2020.


Deficits in fast-spiking inhibitory interneurons (FSINs) within the dorsolateral prefrontal cortex (dlPFC) are hypothesized to underlie cognitive impairment associated with schizophrenia. Though representing a minority of interneurons, this key cell type coordinates broad neural network gamma-frequency oscillations, associated with cognition and cognitive flexibility. Here we report expression of GluN2D mRNA selectively in parvalbumin positive cells of human postmortem dlPFC tissue, but not pyramidal neurons, with little to no GluN2C expression in either cell type. In acute murine mPFC slices the GluN2C/D selective positive allosteric modulator (PAM), CIQ(+), increased the intrinsic excitability as well as enhanced NMDAR-mediated EPSCs onto FSINs. This increase in intrinsic excitability with GluN2C/D PAM was also observed in the Dlx 5/6+/- FSIN developmental deficit model with reported FSIN hypoexcitability. Together these data speak to selective modulation of FSINs by a GluN2D PAM, providing a potential mechanism to counter the FSIN-deficit seen in schizophrenia.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials
  • Adult
  • Animals
  • Female
  • Gene Expression
  • Humans
  • Interneurons / metabolism*
  • Male
  • Mice, Inbred C57BL
  • Middle Aged
  • Neural Inhibition
  • Parvalbumins / metabolism*
  • Prefrontal Cortex / physiology*
  • Pyramidal Cells / metabolism
  • RNA, Messenger / genetics
  • Receptors, N-Methyl-D-Aspartate / genetics
  • Receptors, N-Methyl-D-Aspartate / metabolism*


  • NR2D NMDA receptor
  • Parvalbumins
  • RNA, Messenger
  • Receptors, N-Methyl-D-Aspartate