Zwitterionic polymer chain-assisted lysozyme imprinted core-shell carbon microspheres with enhanced recognition and selectivity

Liwei Qian; Wenqian Liu; Miaoxiu Yang; Valentin Nica; Jinfan Yang; Hanbin Liu; Lulu Ning; Sufeng Zhang; Qiuyu Zhang

doi:10.1016/j.talanta.2020.121085

Zwitterionic polymer chain-assisted lysozyme imprinted core-shell carbon microspheres with enhanced recognition and selectivity

Talanta. 2020 Sep 1:217:121085. doi: 10.1016/j.talanta.2020.121085. Epub 2020 Apr 25.

Authors

Liwei Qian¹, Wenqian Liu², Miaoxiu Yang², Valentin Nica², Jinfan Yang², Hanbin Liu², Lulu Ning³, Sufeng Zhang², Qiuyu Zhang⁴

Affiliations

¹ College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China; School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710072, China. Electronic address: qianliwei@mail.nwpu.edu.cn.
² College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
³ College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address: ninglulu@sust.edu.cn.
⁴ School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an, 710072, China.

PMID: 32498866
DOI: 10.1016/j.talanta.2020.121085

Abstract

Constructing imprinting materials with high recognition and selectivity for protein is an always challenge in protein imprinting technology (PIT). In this work, upon the participating of a zwitterionic polymer chain (Poly (1-vinyl-3-sulfopropylimidazolium), PVSP), a lysozyme imprinted core-shell carbon microsphere (CFC-PVSP@MIPs) was prepared by combining template immobilization method and surface imprinting technology. The carboxyl-functionalized carbon microspheres as substrate provided the CFC-PVSP@MIPs satisfactory adsorption capacity (68.1 mg g^-1), while the dopamine as a functional monomer and crosslinker allowed the imprinted microspheres to have a thin imprinted shell, thus endowing them a fast adsorption equilibrium rate (120 min). In addition, PVSP could be tightly bound to the imprinted layer through non-covalent interaction, which not only simplified the preparation process of CFC-PVSP@MIPs, but also reduced the non-specific adsorption of imprinted material on proteins. Therefore, the resulting CFC-PVSP@MIPs exhibited a more superior recognition ability towards lysozyme with imprinting factor value of 3.10, compared with the PVSP-free imprinted microsphere (imprinting factor value 1.93). Furthermore, benefiting from the characteristics of zwitterionic groups, CFC-PVSP@MIPs also revealed stronger selectivity in competitive adsorption studies of binary protein mixture samples. Consequently, the proposed strategy would be a promising and convenient way to obtain protein imprinted material with high recognition ability, thus would be conducive to further development and application of PIT.

Keywords: Carboxyl-functionalized carbon spheres; Protein imprinting; Surface imprinting technology; Template immobilization; Zwitterionic polymer.