Dek Modulates Global Intron Retention during Muscle Stem Cells Quiescence Exit

Dev Cell. 2020 Jun 22;53(6):661-676.e6. doi: 10.1016/j.devcel.2020.05.006. Epub 2020 Jun 4.


Adult stem cells are essential for tissue regeneration. However, the mechanisms underlying the activation of quiescent adult stem cells remain elusive. Using skeletal muscle stem cells, also called satellite cells (SCs), we demonstrate prevalent intron retention (IR) in the transcriptome of quiescent SCs (QSCs). Intron-retained transcripts found in QSCs are essential for fundamental functions including RNA splicing, protein translation, cell-cycle entry, and lineage specification. Further analysis reveals that phosphorylated Dek protein modulates IR during SC quiescence exit. While Dek protein is absent in QSCs, Dek overexpression in vivo results in a global decrease of IR, quiescence dysregulation, premature differentiation of QSCs, and undermined muscle regeneration. Moreover, IR analysis on hundreds of public RNA-seq data show that IR is conserved among quiescent adult stem cells. Altogether, we illustrate IR as a conserved post-transcriptional regulation mechanism that plays an important role during stem cell quiescence exit.

Keywords: intron retention; muscle stem cells; post-transcriptional regulation; quiescence exit; stem cell quiescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation*
  • Cells, Cultured
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • HEK293 Cells
  • Humans
  • Introns*
  • Mice
  • Mice, Inbred C57BL
  • Oncogene Proteins / genetics
  • Oncogene Proteins / metabolism*
  • Poly-ADP-Ribose Binding Proteins / genetics
  • Poly-ADP-Ribose Binding Proteins / metabolism*
  • RNA Processing, Post-Transcriptional*
  • Satellite Cells, Skeletal Muscle / cytology
  • Satellite Cells, Skeletal Muscle / metabolism*
  • Transcriptome


  • DEK protein, mouse
  • DNA-Binding Proteins
  • Oncogene Proteins
  • Poly-ADP-Ribose Binding Proteins