Isolation, characterization and cytoprotective effects against UV radiation of exopolysaccharide produced from Paenibacillus polymyxa PYQ1

J Biosci Bioeng. 2020 Sep;130(3):283-289. doi: 10.1016/j.jbiosc.2020.05.001. Epub 2020 Jun 4.

Abstract

A novel exopolysaccharide (EPS) from Paenibacillus polymyxa PYQ1 was extracted, well purified and characterized. This EPS was homogeneous glucomannan-type polysaccharide with the average molecular weight of 4.38 × 106 Da. Structural characterization indicated that the monosaccharides of EPS were pyranoses connected by β-glycosidic linkages. Furthermore, our results showed the protective benefits of EPS against UVC induced cytotoxicity in HaCaT cells through scavenging excessive reactive oxygen species, mitigating the decrease of mitochondrial membrane potential, improving catalase activity and maintaining membrane integrity. Taken together, this study qualified EPS from P. polymyxa PYQ1 was a promising natural polymer which worth further investigation as a skin-care agent.

Keywords: Antioxidant activity; Exopolysaccharide; Paenibacillus polymyxa; Purification; Structural characterization; UVC.

MeSH terms

  • Catalase / metabolism
  • Cytoprotection / drug effects*
  • Membrane Potential, Mitochondrial / drug effects
  • Membrane Potential, Mitochondrial / radiation effects
  • Molecular Weight
  • Monosaccharides / analysis
  • Paenibacillus polymyxa / metabolism*
  • Polysaccharides, Bacterial / biosynthesis
  • Polysaccharides, Bacterial / chemistry
  • Polysaccharides, Bacterial / isolation & purification*
  • Polysaccharides, Bacterial / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Ultraviolet Rays / adverse effects*

Substances

  • Monosaccharides
  • Polysaccharides, Bacterial
  • Reactive Oxygen Species
  • Catalase