Prime editing, developed by Anzalone et al., brings genome editing to a new level, because this approach allows introduction of all mutation types, including insertions, deletions, and all putative 12 types of base-to-base conversions. Previously tested in human cells, this technique has been adapted for use in plants by Lin et al.
Keywords: CRISPR-Cas9; custom endonucleases; genome editing; targeted mutagenesis.
Copyright © 2020 Elsevier Ltd. All rights reserved.