In the study, the antimicrobial activities of 7.5% povidone-iodine, 4% chlorhexidine gluconate, 1.5% chlorhexidine gluconate, 1.5% chlorhexidine gluconate with cetrimide mixture, 10% benzalconium chloride, dust detergent and soap as well as changes in activity in due course of time have been investigated. It has been observed that the effect of germicides on Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter cloacae and Candida strains increased in time. Soap had no germicidal effect. The difference between the of antiseptics on the residuent flora of the hands immediately and those two hours later was found to be statistically insignificant.