Environmental cues like noise, pressure, and circadian rhythm can affect the hearing ability of human beings. Nevertheless, the complex physiology of the human being does not allow us to understand how these factors can affect hearing and hearing-related behaviors. Conversely, these effects can be easily checked using the hearing organ of Drosophila melanogaster, the Johnston organ. In the current study, the Drosophila was exposed to challenging environments like noise, low pressure, and altered circadian rhythm. The hearing organ of larvae, as well as adults, was analyzed for hearing-related defects. In the third instar larva, the cell deaths were detected in the antenna imaginal disc, the precursor of Johnston's organ. Elevated levels of reactive oxygen species and antioxidant enzymes were also detected in the adult antennae of environmentally challenged flies. The ultrastructure of the antennae suggests the presence of abundant mitochondria in the scolopidia of control. Fewer amounts of mitochondria are found in the environmentally challenged adult antennae. In adults, various hearing-related behaviors were analyzed as a readout of functionality of the hearing organ. Analysis of climbing, aggressive, and courtship behaviors suggests abnormal behavior in environmentally challenged flies than the control. The current study suggests that the environmental cues can alter hearing-related behaviors in Drosophila. The methods used in this study can be used to monitor the environmental pollution or to study the effect of alteration of noise, pressure, and circadian rhythm on hearing-related behaviors taking Drosophila melanogaster as a model organism. Graphical abstract.
Keywords: Antioxidant enzymes; CR alteration; Drosophila; Johnston’s organ (JO); Low pressure; Noise.