doi: 10.1007/978-1-0716-0585-1_3.
Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration
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- PMID: 32524470
- DOI: 10.1007/978-1-0716-0585-1_3
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Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration
Methods Mol Biol.
2020.
Abstract
Primary cultures of neurons of the peripheral nervous system have been successfully used for studying many aspects of neuronal development and survival, including investigations into the mechanisms of axon degeneration. In this chapter, we describe how to prepare and microinject dissociated cultures of sympathetic neurons of the superior cervical ganglion (SCG) specifically for use in highly controlled and targeted assays of axon survival and degeneration.
Keywords: Axon degeneration; Axon survival; Axon transection; Dissociated SCG culture; Microinjection; Superior cervical ganglion; Wallerian degeneration.
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