Genome sequence analysis of a Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) isolated from Heliothis peltigera in Turkey

PLoS One. 2020 Jun 12;15(6):e0234635. doi: 10.1371/journal.pone.0234635. eCollection 2020.

Abstract

The entire genome of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV-TR) was sequenced, and compared to genomes of other existing isolates. HearNPV-TR genome is 130.691 base pairs with a 38.9% G+C content and has 137 open reading frames (ORFs) of ≥ 150 nucleotides. Five homologous repeated sequences (hrs) and two baculovirus repeated ORFs (bro-a and bro-b) were identified. Phylogenetic analysis showed that HearNPV-TR is closer to HaSNPV-C1, HaSNPV-G4, HaSNPV-AU and HasNPV. However, there are significant differences in hr3, hr5 regions and in bro-a gene. Pairwise Kimura-2 parameter analysis of 38 core genes sequences of HearNPV-TR and other Helicoverpa NPVs showed that the genetic distances for these sequences were below 0.015 substitutions/site. Genomic differences as revealed by restriction profiles indicated that hr3, hr5 regions and bro-a gene may play a role in the virulence of HearNPV-TR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Circular / genetics
  • Genes, Insect
  • Genome, Viral*
  • Moths / virology*
  • Nucleopolyhedroviruses / genetics*
  • Nucleopolyhedroviruses / isolation & purification*
  • Open Reading Frames / genetics
  • Phylogeny
  • Restriction Mapping
  • Sequence Analysis, DNA*
  • Turkey

Substances

  • DNA, Circular

Supplementary concepts

  • Helicoverpa armigera nucleopolyhedrovirus

Grants and funding

This study was financially supported by the Scientific and Research Council of Turkey (TUBITAK) (Project No: 118Z003). The grant was received by Gozde Busra Eroglu. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.